LOCUS       sequence-50181-e        4767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00712
FEATURES             Location/Qualifiers
     source          1..4767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4722..4730
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggatcgtc gtgcattgaa gtttatgcaa aaaagagcgg acacagaaag cgatacgacc
       61 acacccgtga gcaccacagc atcccagggg atttcagcat cagcgattct agcaggaggc
      121 actcttccct tgaaggacaa ctcgaacatc cgcgagaagc ccctccacca taactacaac
      181 cacaataaca acaacagctc ccagcactca cactcgcacc agcagcagca acaacagcag
      241 gtgggtggca agcagctcga gcggccacta aagtgcctgg aaacgctcgc ccagaaggcg
      301 ggaatcacct tcgacgagaa atacgatgtg gccagccccc cgcatcccgg cattgcccag
      361 cagcaggcga cttcaggaac aggcccagca acgggatcag gctcagtcac ccccacaagc
      421 catcggcacg gaactccgcc cacgggccgc aggcaaaccc acaccccaag cactccgaac
      481 aggcccagtg ctcccagcac acccaacact aactgcaact caattgcccg ccacaccagc
      541 ctcacgctgg agaaggcgca gaatcccggc cagcaggtgg ccgccaccac cacggtgcca
      601 ctgcagatat cccctgagca gctgcagcag ttctatgcga gcaatcccta cgccattcag
      661 gtgaagcaag agtttcccac gcacacgacc agtggcagtg gaactgaact aaagcatgca
      721 accaacatta tggaagttca gcagcagttg cagctgcagc agcagctgtc ggaagccaac
      781 ggtggaggag cagcctcggc cggagccgga ggagcagcta gtccggccaa ctcgcagcaa
      841 agccagcaac agcagcactc cacagccatc agcaccatgt cgccgatgca attggcagcg
      901 gccactggag gagttggcgg ggattggaca cagggaagga cggtgcagct aatgcaaccc
      961 tccaccagtt tcctgtatcc ccaaatgatt gtgtcgggaa atctgttgca tccaggaggc
     1021 ctcggtcagc agccaatcca ggtgatcacc gccggcaagc cattccaagg caacggcccc
     1081 cagatgctta ccaccacgac tcaaaacgcc aagcaaatga tcggtggcca agcgggattc
     1141 gctggcggaa attacgcgac ctgcattccc acaaaccaca atcaatcgcc ccagacggtg
     1201 ctcttctcac cgatgaacgt catttcgcca cagcagcaac agaacctgct gcaatcaatg
     1261 gccgctgcag ctcagcagca gcaactcacc caacagcagc aacagtttaa ccagcagcaa
     1321 caacagcagc ttactcagca gcaacagcag ttgacagctg ctctggccaa ggtgggagtg
     1381 gatgcgcagg gcaagctggc ccagaaagtg gttcagaaag tgactaccac cagtagcgcg
     1441 gtccaggcgg cgacgggtcc tggatctact gggtcaacac agacccagca ggtgcagcag
     1501 gttcagcaac agcagcagca gaccacccaa accactcagc agtgcgtgca ggtttcacag
     1561 tcgactttgc cagtcggtgt gggtggacag tctgttcaga ctgcccaact tctgaacgct
     1621 ggccaagcgc aacaaatgca gattccctgg ttcttacaga atgctgcagg actgcagccg
     1681 tttgggccaa accagatcat cctgcgaaac cagccagacg gaacccaagg catgttcatt
     1741 caacagcaac cggcgacgca gactttgcag acccagcaaa accagattat tcaatgcaac
     1801 gtgacgcaga cgcccactaa ggcacgcact caactggatg cacttgctcc caagcagcaa
     1861 cagcagcagc agcaggttgg cactaccaac cagacgcagc agcagcaact agcggtggct
     1921 actgcccagt tgcagcaaca gcagcagcaa ctcactgcag cagctctgca gcgaccagga
     1981 gcccctgtca tgccccacaa tggaactcaa gtgcgtccgg ccagttccgt atccacacag
     2041 actgcccaga accagagcct gctgaaggcc aaaatgcgca acaagcagca gccggtgcgc
     2101 cccgctttag ccacattgaa aaccgaaatc ggtcaagtcg caggacaaaa taaggtagta
     2161 ggccacctga ccaccgtgca gcagcagcaa caggcgacga atctccagca ggtggttaat
     2221 gcggcgggca acaaaatggt tgtgatgagc acaacgggca ctccgatcac cctgcagaat
     2281 ggacagaccc ttcatgcagc cactgcggca ggagtcgaca agcagcaaca gcagctacaa
     2341 ctgtttcaga aacagcaaat cctgcaacaa caacaaatgt tgcaacagca gattgctgcc
     2401 attcaaatgc agcagcagca agcggctgtt caggcccagc aacaacagca gcaacaggtc
     2461 tctcagcagc agcaggttaa cgcccagcaa cagcaagcgg tggcgcaaca acaacaggca
     2521 gtcgcgcagg ctcagcaaca gcagagggag caacagcagc aagttgccca agcccaggcg
     2581 cagcatcaac aggctctcgc gaatgccact cagcaaatcc ttcaggtggc gccaaatcaa
     2641 ttcatcacgt cccaccagca acagcagcag cagcaacttc acaaccaact gatacagcag
     2701 cagctacagc aacaggcgca ggcacaagtt caagcccaag tgcaggctca agcgcaacag
     2761 caacaacagc agcgagagca gcagcagaat attatccagc agattgtggt gcaacagtct
     2821 ggagcgactt ctcaacagac ttcccagcag caacagcacc accaatccgg gcaactacag
     2881 ctaagtagcg tgcccttctc agtttcttcg tcaacgacgc cagccggaat agctacctct
     2941 agtgctctgc aggcagccct ctccgcctct ggcgccatct ttcagacagc taagccgggt
     3001 acttgcagtt cctcctcccc cacaagcagt gtggtcacaa ttaccaacca gagcagcaat
     3061 cctttggtca ccagcagtac ggtggccagt atacagcagg ctcagacgca atctgctcag
     3121 gtccaccaac atcagcagct aatcagcgcc acaattgccg gagggactca acaacagcca
     3181 cagggaccgc catcacttac acccaccaca aatccaattt tggccatgac ctcgatgatg
     3241 aatgctacag tgggtcacct ttccactgct ccgcctgtaa ctgtttctgt gacaagcacc
     3301 gctgttactt cgtcgccggg tcagctggtt ctcttaagca cggctagtag cggtggagga
     3361 ggtagcatac cagccacgcc caccaaagag acaccttcga aagggcccac cgcaaccctg
     3421 gtgcccattg gttcgcccaa gactcctgta tcaggaaagg acacctgcac tacccccaaa
     3481 tcatctactc ctgccactgt cagcgcatcc gtagaggcca gtagttccac aggcgaagcc
     3541 ctgtccaatg gagatgcctc agataggtct tccacgccgt caaagggcgc taccactccc
     3601 accagcaagc aaagcaatgc agcagtgcag ccaccgagta gcaccactcc caacagtgtc
     3661 agtgggaaag aagagccgaa gctggcaacc tgcggcagtt taacgtccgc aacatcaact
     3721 tcaaccacga caacgatcac caatgggatt ggagtagcca gaacgacagc cagcacggct
     3781 gtctcaaccg ctagcacaac cactaccagt tctggcacct ttatcacaag ttgcaccagc
     3841 acaaccacaa ccaccacgtc gagtatcagt aatggatcga aggatctccc caaggcgatg
     3901 attaagccga acgtcttaac tcacgtcatc gatggcttca tcatccagga ggccaacgag
     3961 ccatttcccg tcaccagaca gcgatatgca gacaaagacg tcagcgatga gccgccaaag
     4021 aaaaaggcaa ccatgcagga ggacatcaag ctaagtggaa tagcatcagc tccaggctcg
     4081 gatatggttg cttgcgagca gtgtggaaag atggagcaca aagcaaagct gaaacggaag
     4141 cgctactgtt cgccaggatg ctcgaggcag gcaaagaacg gcatcggtgg agttggatca
     4201 ggagagacga acggcctggg gacaggtggt atagttgggg tggacgccat ggcattggtg
     4261 gacaggctgg atgaagccat ggctgaggag aagatgcaga cagaggccac cccaaagctt
     4321 tcagaatcgt ttcctatttt gggagcctca acagaagtac ctccaatgtc actgccagtc
     4381 caagcggcga tttctgcgcc ctcgcctctt gcaatgcctc taggatcgcc attgtcagtt
     4441 gcacttccaa ctcttgcacc actgtctgta gtcacttctg gcgcggcgcc caagtcttcg
     4501 gaagtgaatg gaacagatcg tccgccaatc agcagctgga gtgtggacga tgtcagcaac
     4561 ttcattcgag aactgcctgg ttgtcaggac tacgtggacg actttataca gcaggagatc
     4621 gacggccaag cgcttctgtt gctcaaggag aagcatttgg tgaacgctat gggcatgaag
     4681 ctgggtccag ctcttaaaat tgtggccaag gtggagtcca ttaaggaggt cccgccacct
     4741 ggcgaggcca aggatccagg agcgcag
//
