LOCUS       sequence-50188-e        5963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00727
FEATURES             Location/Qualifiers
     source          1..5963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..17)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    1972..1983
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     regulatory      2905..2914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3189..3200
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggacctgc cttcaatggt gcagcgttca ggagacacgc tcatcgtgcg cagcgtggtc
       61 agcggcaatc agctgtacac ggagcagggt ggccaccagc ccaccagtgg gaacacggga
      121 cacatgacga gcaactcgaa cagcaatacg ccagtggcac aggcgaactc atcccttttg
      181 gagcgacatg tggagcgttt ccggctgcaa cagttgctgc aacaacagcg ccaagcagca
      241 gctgcagttg ccgttgtgaa cagcgtgcag cagcagcagc aacagcagcc acagcaggtc
      301 ggcatggatg ccaaggagga gggcctgccg cagtgcaaga tcaagcggaa ctatagctgc
      361 aaccactgtg cgtacttcac acagaatccg cgctatcact tgacccatct gcgcgatgtg
      421 cacggcgaga agatagtgat caacaagtgc aagctgtgcc tgtacgcatc gcgacatttc
      481 cagaagctgg tgcggcacat gaaaatggtg cacggctgca cggacggcat tcccagtggt
      541 catggtcagg ccaggggcaa gaggggcatg agccgggagg cacgcaagcg ccggctggag
      601 gagagcgtgg gtgtaatggg tggccaatcc ctgacagtca ccgttccgga tgttcccacg
      661 ctggagcaag tgaagcgaga gctgctcctg caggaggaga agctgcagcg ggacattcag
      721 gccttcaacc agcgacagcg cgaggagcag cagcgcgaac agcagcgtga actggaactg
      781 gtggccacca gtgcgtatga gcgacaaatg caggtccttc gcgactacga gcgtcagtcg
      841 cccgccgaac caccaactcc ttcgcctacc agcggatcgg caacgccccc ctccaacggg
      901 gaggagcccc agaaccggct gctcaagtgt agcgcctgcg agttcaccac cctctatcga
      961 acccaactgc gtgcccacga gctggccgag cacggcaaga ccaagttctt ccgctgcgac
     1021 aagtgcagct atgtgaccca catcaaggcg cgctttagca agcacgtcaa gtaccactcc
     1081 atgccgatga tcaagtgcgt cacctgcgac ttccgcacgc cctacaaatg gaatctggac
     1141 aggcacatga agaatcatgg cggcgcggga gccttcaagt gcgccgcttg cgactttacc
     1201 gctgacatca agcaatcgct gacggtccac gagatgaacc accatgtgcc gccggtgggc
     1261 aatgccggct ccatttggcc aagacgccag aataaagtcg gagcgagcga gatgtgcgag
     1321 gatttcctca gcgattccgc cgaactggag gatcaatata acaacaacaa cgtcgatgac
     1381 gaactggacg gcgtcgatga tgccgacgag gcaatgagtg gaggggagga gcaaccgttg
     1441 cacctccatc actacggcaa acggagcaag tacgacgatg aggaggagcc cacagatctc
     1501 tcgcagaagg gcggatgctc ctcggacacc tccagtgtgg gaacgcccac gccgagggca
     1561 cagcgaacca tgcccaatct cataccgatc agcaaggggg ccaaagacgt attaaacctt
     1621 tcgaaggaga cgaatgcctc gcgcagttcc ctgacggaga tcgcctcgat gttcttcaat
     1681 gagaaacaga tctcggagat gcttgacaag tcggatgtgc cacaattgtc gccggcaacg
     1741 actgtggcct cccagaactc gacgcgcagc caattgacca agaaatccag tttcatggac
     1801 aaactgaaga cgggggcgca gcacgagaat ctggtgtgcc agtgcggtca tgtggccaag
     1861 tgcctttcgg agtcgatcat ccacggcaag agctgtcacg cctcggcggt gatcatcgac
     1921 gaggatgaag ctggtctgca cgaggacgat ggcgacgatc ggctggagat cgatgaggat
     1981 gacgaagatc accactcgca ttcggccctg aatctcagtg tgacgggatc gacgcgttgc
     2041 cagcactgcc ggcaccgctg caagtcctcc acagatctga tgcaccatct gaagcagtgc
     2101 gtcgaggcga tccgttgcgc caacgagatg tacgactcga attccggcga gagtggcgat
     2161 cggcgaccgg actcgcagag cctccagcag caggcggccg tccagcagca gcgggtgtgc
     2221 atctggaaca aggccaccaa ggagatcgtc gccgcagcgg ccgcatccct acagcagaac
     2281 aagaacaacc acaacctggt taagtcccca accggaagtc aggcggccag taacgaggag
     2341 aacagctact atggcgtaga aacggcaccc ggatacggcg agatgacacc ggaggaagag
     2401 gcagccaact cctcgctgaa gaaggtgtac aagtgtccgc actgcagctt ctgggcctcc
     2461 acggcgtccc gcttccacgt ccacatcgtg ggccatctga acaagaagcc ctttgagtgc
     2521 tccctctgct cgtaccgctc caactggcgc tgggacatca ccaagcacat ccgattgaag
     2581 acgatccgcg atccctcgca caagacggcc aaggtactga tgaacgacga gacgggccgc
     2641 cggaactaca ccaagtacaa caagtacatc accctgatga aggtaacaga ggaggatggt
     2701 gatcccaagc tgatgaagtc cggcgagatg acaccgaacc aggtggcctc gttggccttc
     2761 ctcaaggact atgccaaggt gggctcggtt tccgggcagg atatcacatt ggaaccagtg
     2821 cttcccaaca aacccaatgt actggacgat gcccatttgg cggacaacct tatacggatt
     2881 cccttgctgg ccaccatgat gaatgccgcc atggcgcaac agcaacacca gcagcagcag
     2941 cagaaggagc atcagtccac gatgatcacg ccctctgtga ccatttcccc ggtgaagcga
     3001 tccaatcagg catccgttat gcagggcaag cccagcgacg acctgatcac cgaggtccac
     3061 caggagggca atgagaagag gaccgtctat cggtgccgca agtgtaactt cgggcaccag
     3121 aatcgcgatg ctgtgctggc gcacgtaaag attcactacc aggacgccag ctatccgaag
     3181 tccaactctg ggaactccag tacttcgcca ctgcaggtat ccgtgggcgg aagtccacaa
     3241 ttctacatga acaaggtctt tgccgccatg tgcctgtcgc agacccagtc acagtcgtcg
     3301 cccaactccg gctctgccgg aacaagtccg gccatctcgg ccggtctgct gcagcgggcc
     3361 atacaggagg cccagcactc gccaactccg aacgccagcg ctctaagcgg acttgctttg
     3421 gcgttggcgg gcggaaagcc acaagccaat acgacgaaag ccagtgccgc ctccatttta
     3481 gagcacggtg agcagaaagc gagtcaaaac gaggcaagtg ccgacagtct gacgtcgccg
     3541 aacaccacca ccacgaactc aaccactagt agaaccacca aagacaccac cgccagatcg
     3601 ctgcaggatc tgctcacctc accgcgcagt gccagaaatg gaaatcatcc ttctaacgct
     3661 aacagtaact cggttgtggg aaatggactc cggctggatg ccgtctacgt cgcctcatcc
     3721 accaacaata ccacaacacc accaccacct cccaccaact cagtcagcaa gcccaatgca
     3781 tcgcctttgc cacggcctgt cgcttaaagc attccgggga tattcggatc gagacccttg
     3841 agcgaggagc cagtgtctcg gcgaacgcac cacccatcta ccgacccctg ggagcaggtg
     3901 ccagcaacga atcccagtcc cacatccaca atccgtcgaa tcccatcacc agcaacatcc
     3961 tgaagtccgg cagcaacacg aatgccaaca gcaacatcaa cagcaatagt gcgcagcccc
     4021 tgctgctgac caccaagcag ctggagcaga tcttccacac accgctctcc gccagcgccg
     4081 cagccgtggt gaatgcggcc aacacccagc aggatatcca agcggcggca gcctattggg
     4141 cagcagcctg caaagcagtg gtggccaaca gcagcgccga ggagttgctg cagttgcagc
     4201 agaatgacca gatcgagata acccgcctgc cgaccacgac agagcactcc atcggatcca
     4261 gcggcaacaa taccaagagc aagcagcaaa agtgtccggt ctgtccgtac atctcggaga
     4321 gcaaatccca gatgaactac catgtgtcgc tgcacaagcc cacgcaatac gagtgccgcc
     4381 tgtgcacatt tgtgtgtgcc aaaaagcagc atctgagcag ccacatgaga acggtgcacc
     4441 agcagcagat ggtggcatcg ggagcaggtg cagccgcagg agcagccgga ggagtaggag
     4501 gcggcggagc tgcctcactg ggtctggact ttagtgtggc cctgcagttg gccgcagctg
     4561 ccaagcaggt gcaacaactg cccgcatcaa caccgctctc catcgatttg tcccaaattc
     4621 agatggatgc cgcctccgat gcggagctca atccacagca actgccgccg gagtaccagt
     4681 acaagctgat tagttactgt cccaggtgtc cggctcgctt tgcccagaag cacaatgacg
     4741 agcgcaatgc caagcaagag ctagagcagc atttgctggc ccacagcgat caggagccgg
     4801 aggatcagga cccgagctat gtctgcgcct attgtgagta tcgtacgtcg gaggagacta
     4861 tgctccaact gcaccgtgcg gtgcacatgt cccactacca ggagaagtgc caggagctct
     4921 ataggaactg caaggaggat gtcgagtatc cggcacccaa gttgctgcag ctgactggtc
     4981 ccgaaaccat ttgggtggtg gataacgaat tgagcttgca actgctgcag cagactaccg
     5041 gcggtgcagt tagtacctcc accggtgggt ttgacaacca gaactctctg ctcaagaagc
     5101 agctggaatc gggcggtggc cagatggtcc cacttccccg ggaagtatcg gtgaacaaaa
     5161 acgaggccga agcccccgag gaggacgagg agcgacatag cagctccaca ccgtcgacca
     5221 gcgcttcggt ggccaccagt gatttggccg cagatggcag cagtgatgca ggatccgttg
     5281 acatgcccca gtccattccg gcaccggaac gctgtctcca ctgtccattc gagacgcagc
     5341 agcacgagga gctgatgcag catctacaga agcacgcctg cgtaaatcct ccacctccca
     5401 actgccagaa atgtgcccat tgtgattaca atgccaatga ggaggcggaa atggaggagc
     5461 acacggcgct gcatttcaat gctggtgaga agctcaagtc cgtggagttc ttcacctgct
     5521 acgatcagct ggagatcagc gtggagcggg aaccggagga gaccgctgac tcaaaggtgg
     5581 agcagaccga gcacaacaac aaccaggaca atgtgatgaa cgccaatgtg cagcagcagc
     5641 agcaacagca gccaagcagc agcggcccag atgaacagcc agctgtcgag ggaatggagg
     5701 agtcgtcggc caaaaagtcc agcacaaagt tgattctgta caagaacgat ggcggcttga
     5761 gtgtgaagct ctcccaggat gagggcgcca attcagagtc ccagaagagc gagaatatca
     5821 gcgatcgcct gcgcagaagg atccttcggg gaagtgccaa ccagtcggag gacgccactt
     5881 ccacctccgg ccaggagcga cccgcaacac cggaaaaaat gatactggtc aatgcccaga
     5941 cgggcaaagt catttccaga aag
//
