LOCUS       sequence-50198-e        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00752
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1453..1470
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atgtttccga accagaacaa tggagccgct cctgggcagg gtccagcagt tgatggccaa
       61 cagagcctca actacaacgg cctgcccgcc cagcagcagc agcaattggc acagtccacg
      121 aaaaatgtgc gaaagaagcc ctacgtaaag atcaccgaac aaccggcagg aaaggcgcta
      181 aggtttcgct acgagtgcga gggacgctcg gcgggatcca ttccgggcgt gaactctacg
      241 ccggagaaca agacctatcc gacaatcgaa attgtgggct acaagggacg cgccgtagtt
      301 gttgtctcct gcgtcacaaa ggatacgcca tatcgtcctc atccccacaa tttagttggc
      361 aaggagggct gcaagaaggg cgtctgtaca ctggagatca acagtgagac aatgcgagcg
      421 gtgttcagta acttgggtat ccagtgtgtc aaaaagaagg acattgaggc ggcgctcaag
      481 gcgcgcgagg agatccgtgt ggatccgttt aagactggct tttcgcatcg tttccagccc
      541 tcgagcatag atctgaattc ggtgcgattg tgctttcaag tattcatgga gagcgagcag
      601 aagggtcgat tcacctcgcc actgccgccg gtagtttcgg agcccatctt cgataagaag
      661 gccatgtccg acctggtcat ctgccggctg tgtagctgct cggccaccgt tttcggcaac
      721 acccagatca tcctgctctg cgagaaggtg gccaaggagg acatctccgt gcgattcttt
      781 gaggagaaga atggccagag tgtttgggag gcctttggtg acttccagca cacggatgtc
      841 cacaagcaga ctgccattac ctttaagacg ccgcgctatc ataccctgga catcacagag
      901 cccgccaagg tttttattca actgcgacgt ccctcggatg gagttaccag cgaggccctg
      961 cccttcgagt acgtgccaat ggactcagat ccagcgcact tgaggcggaa acgtcagaag
     1021 actggcggtg atcccatgca cctgctgctc cagcagcagc agaaacagca attgcagaat
     1081 gaccaccagg atggcagaca aactaacatg aattgctgga atacacaaaa cataccgccc
     1141 attaaaacgg aaccaagaga tacctcgcca caaccctttg ggctttctta tcgggcgcct
     1201 cctgagctca caccctcgcc gcagccgctg tcgccatcga gcaactacaa ccacaacagc
     1261 acgccctcgc cctacaacat ggcctccgcg gtcacgccca ccaatggcca gcagcaactg
     1321 atgtcgccca accatccaca gcagcaacag caacagcagc aatatggagc aactgatctg
     1381 gggagcaact acaatccgtt tgcccagcag gtccttgccc agcagcagca gcatcagcag
     1441 cagcagcaac agcaccagca tcagcaccag cagcaacacc aacagcagca gcagcagcag
     1501 cagcagcagc aacagcaaca gtccttgcaa tttcatgcca atccctttgg caatcctggc
     1561 ggcaatagct gggaaagtaa attctcggcg gcagctgttg cagcagcggc agcaactgcg
     1621 acaggagcag cacctgccaa cggcaatagc aataatctca gcaatctcaa caatcccttc
     1681 accatgcaca acctgctgac atcgggcgga ggtcctggca acgccaacaa tctgcagtgg
     1741 aacctgacca caaatcatct gcacaatcag catacgcttc atcagcagca gcagcttcag
     1801 cagcagcagc agcagcagta cgacaatact gcgcccacca acaacaatgc caaccttaat
     1861 aacaataata acaataataa taccgctggc aatcaagcgg ataataatgg gccaacgctc
     1921 agcaatctgc ttagcttcga tagcgggcag ttggtgcaca taaactcaga ggatcagcag
     1981 atactgcgcc tcaattcgga agatctgcag atatcgaacc tgtccatatc cacg
//
