LOCUS       sequence-50209-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Koutelou E, Sato S, Tomomori-Sato C, Florens L, Swanson SK, Washburn
            MP, Kokkinaki M, Conaway RC, Conaway JW, Moschonas NK
  TITLE     Neuralized-like 1 (Neurl1) targeted to the plasma membrane by 
            N-myristoylation regulates the Notch ligand Jagged1.
  JOURNAL   J Biol Chem. 2008 Feb 15;283(7):3846-53. doi: 
            10.1074/jbc.M706974200. Epub 2007 Dec 12.
  PUBMED    18077452
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M706974200"; journalName: "J Biol Chem"; date: 
            "2008-02-15- 15"; volume: "283"; issue: "7"; pages: "3846-53"
COMMENT     Sequence Label: Jag1-HA pIRES
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             284..373
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    451..572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(462..481)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     516..535
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(844..863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cacagtcccc atcaaggatt atgagaacaa aaactccaaa atgtcaaaaa tcaggacaca
       61 caactcggaa gtggaagagg atgatatgga taagcaccag cagaaagtcc ggtttgccaa
      121 acagcccgtg tacacgctgg tagacagaga ggagaaggcc cccagcggca cgccgacaaa
      181 acacccgaac tggacaaaca aacaggacaa cagagacttg gaaagtgccc agagcttgaa
      241 ccggatggag tacatcgtaa tccgcggtgg cggccgcatc ttttacccat acgatgttcc
      301 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
      361 tccagattac gctgctcagt gcggccgctc tagagtcgac ccgggcggcc gcttcccttt
      421 agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
      481 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      541 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      601 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
      661 gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
      721 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
      781 agcgcgcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      841 ccgctccttt cgctttcttc ccttctt
//
