LOCUS       sequence-50225-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX2
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..82
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             520..537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(590..608)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      604..651
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(775..794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag atctgggtac
       61 cgagaacctg tacttccaat ccatggagag caatacatca tcatctttgg agaatttagc
      121 gacggcgcct gtgaaccaga tccaagaaac aatttctgat aattgtgtgg tgattttctc
      181 aaaaacatcc tgttcttact gtacaatggc aaaaaagctt ttccatgaca tgaatgttaa
      241 ctataaagtg gtggaactgg acctgcttga atatggaaac cagttccaag atgctcttta
      301 caaaatgact ggtgaaagaa ctgttccaag aatatttgtc aatggtactt ttattggagg
      361 tgcaactgac actcataggc ttcacaaaga aggaaaattg ctcccactag ttcatcagtg
      421 ttatttaaaa aaaagtaaga ggaaagaatt tcagtgacag taaaggtgga tacggatccg
      481 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      541 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      601 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      661 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      721 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      781 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      841 ggggggctcc ctttaggggt tccgatttta gtgctttacg ggcacctcg
//
