LOCUS       sequence-50226-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             728..745
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(798..816)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      812..858
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgatt gccaagtcct
      121 tctatgacct cagtgccatc aacctggatg gggagaaggt agatttcaat acgttccggg
      181 gcagggccgt gctgattgag aatgtggctt cgctctgtgg cacaaccacc cgggacttca
      241 cccagctcaa cgagctgcaa tgccgctttc ccaggcgcct ggtggtcctt ggcttccctt
      301 gcaaccaatt tggacatcag gagaactgtc agaatgagga gatcctgaac agtctcaagt
      361 atgtccgtcc tgggggtgga taccagccca ccttcaccct tgtccaaaaa tgtgaggtga
      421 atgggcagaa cgagcatcct gtcttcgcct acctgaagga caagctcccc tacccttatg
      481 atgacccatt ttccctcatg accgatccca agctcatcat ttggagccct gtgcgccgct
      541 cagatgtggc ctggaacttt gagaagttcc tcatagggcc ggagggagag cccttccgac
      601 gctacagccg caccttccca accatcaaca ttgagcctga catcaagcgc ctccttaaag
      661 tttgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      721 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      781 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaacgg
      841 gtcttgaggg gttttttgct gaaaggagga actatatccg gat
//
