LOCUS       sequence-50227-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-C 16E7
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..39)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(13..39)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(55..78)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(426..450)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(479..498)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(480..498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(526..542)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(558..580)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ggtagaattc taggcgtagt cgggcacgtc gtaggggtat cctccagcgg ccgacttgtc
       61 atcgtcgtcc ttgtagtcct cgagttcgaa ccgcgggccc tctagatcaa ccactttgta
      121 caagaaagtt gatggtttct gagaacagat ggggcacaca attcctagtg tgcccattaa
      181 caggtcttcc aaagtacgaa tgtctacgtg tgtgctttgt acgcacaacc gaagcgtaga
      241 gtcacacttg caacaaaagg ttacaatatt gtaatgggct ctgtccggtt ctgcttgtcc
      301 agctggacca tctatttcat cctcctcctc tgagctgtca tttaattgct cataacagta
      361 gagatcagtt gtctctggtt gcaaatctaa catatattca tgcaatgtag gtgtatctcc
      421 atgcatgcca acttttttgt acaaacttgt tgatagctta actagccagc ttgggtctcc
      481 ctatagtgag tcgtattaga tctaattccg gcgcctagag aaggagtgag ggctggataa
      541 agggaggatc gaggcggggt cgaacgagga ggttcaaggg ggagagacgg ggcggatgga
      601 ggaagaggag gcggaggctt a
//
