LOCUS       sequence-50228-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX2
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(657..677)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(702..719)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             730..752
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    767..791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(791..810)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(792..810)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatccg tatccacctt tactgtcaaa ctttaaggag gcgcttgatg
      121 tcaggctcaa tgttgatggt tgggaaggtg cggctgtagc gtcggaaggg ctctccctcc
      181 ggccctatga ggaacttctc aaagttccag gccacatctg agcggcgcac agggctccaa
      241 atgatgagct tgggatcggt catgagggaa aatgggtcat cataagggta ggggagcttg
      301 tccttcaggt aggcgaagac aggatgctcg ttctgcccat tcacctcaca tttttggaca
      361 agggtgaagg tgggctggta tccaccccca ggacggacat acttgagact gttcaggatc
      421 tcctcattct gacagttctc ctgatgtcca aattggttgc aagggaagcc aaggaccacc
      481 aggcgcctgg gaaagcggca ttgcagctcg ttgagctggg tgaagtcccg ggtggttgtg
      541 ccacagagcg aagccacatt ctcaatcacc acggccctgc cccggaacgt attgaaatct
      601 accttctccc catccaggtt gatggcactg aggtcataga aggacttggc aatcatggat
      661 tggaagtaca ggttctcggt acccagatct acaccagaag aatgatgatg atgatggtgc
      721 atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg
      781 ctcacaattc ccctatagtg agtcgtatta attttcgcgg gga
//
