LOCUS       sequence-50231-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RHOD
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             741..758
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(811..829)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tggcgggtga ggaggcgcca
      121 ccaggcgtgc ggtccgtcaa ggtggtcctg gtgggcgacg gcggctgcgg gaagacgtcg
      181 ctgctgatgg tcttcgccga tggggccttc cccgagagct acacccccac ggtgtttgag
      241 cggtacatgg tcaacctgca agtgaaaggc aaacctgtgc acctccacat ctgggacaca
      301 gcagggcaag atgactatga ccgcctgcgg cccctgttct accctgacgc cagcgtcctg
      361 ctgctttgct tcgatgtcac cagcccgaac agctttgaca acatctttaa ccggtggtac
      421 ccagaagtga atcatttctg caagaaggta cccatcatcg tcgtgggctg caagactgac
      481 ctgcgcaagg acaaatcact ggtgaacaag ctccgaagaa acggattgga gcctgtgacc
      541 taccacaggg gccaggagat ggcgaggtcc gtgggcgcgg tggcctacct cgagtgctcg
      601 gctcggctcc atgacaacgt ccacgccgtc ttccaggagg ccgccgaggt ggccctcagc
      661 agccgcagtc gcaactgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
      721 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      781 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      841 ggg
//
