LOCUS       sequence-50235-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 233
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(93..803)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(173..196)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(371..390)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     640..658
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             810..821
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcaccgacaa acaacagata aaacgaaagg nncngnnttt cgactgagcc tttcgtttta
       61 tttgatgcct ctagcacgcg tacnatgggc ccttacttgt acagctcgtc catgccgccg
      121 gttgagtggc ggccctcggc gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg
      181 gaggtgatgt ccagcttgat gttgacgttg taggcgccgg gaagctgcac gggcttcttg
      241 gccttgtagg tggtcttgac ctcggcgtcg tagtggccgc cgtccttcag cttcagcctc
      301 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
      361 cagcccattg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
      421 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
      481 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      541 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
      601 ggggacagga tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttggcg
      661 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
      721 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
      781 tcctcctcgc ccttgctcac catcgtacgt ttctcctctt taataagctt aagaccact
//
