LOCUS       sequence-50236-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PICK1
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             332..349
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(402..420)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      416..463
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(588..607)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggaaggtgac cctgcagaag gatgctcaga acctgatcgg gatcagcatt ggaggagggg
       61 cccagtactg tccctgcctc tatatcgtcc aggtatttga caacacccca gcagccttgg
      121 acggcacagt ggcagctggc gatgagatca ccggtgtcaa tggcaggtca atcaaaggga
      181 aaactaaggt ggaggtggcg aagatgattc aggaggtgaa gggggaggtg accatccact
      241 acaacaagct gcagtattac aaggtttgac agtaaaggtg gatacggatc cgaattcgag
      301 ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      361 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      421 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      481 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcgnc gggtgtggtg
      541 gttacgcgca gcgtgaccgc tacacttgcc agcgccccta gcgcccgctc ctttcgcttt
      601 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggc
//
