LOCUS       sequence-50237-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PICK1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(383..403)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(428..445)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             456..478
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    493..517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(517..536)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(518..536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(620..639)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttgtcgacgg agctcgaatt cggatccgta tccaccttta ctgtcaaacc ttgtaatact
      121 gcagcttgtt gtagtggatg gtcacctccc ccttcacctc ctgaatcatc ttcgccacct
      181 ccaccttagt tttccctttg attgacctgc cattgacacc ggtgatctca tcgccagctg
      241 ccactgtgcc gtccaaggct gctggggtgt tgtcaaatac ctggacgata tagaggcagg
      301 gacagtactg ggcccctcct ccaatgctga tcccgatcag gttctgagca tccttctgca
      361 gggtcacctt cccaggcacc atggattgga agtacaggtt ctcggtaccc agatctacac
      421 cagaagaatg atgatgatga tggtgcatat gtatatctcc ttcttaaagt taaacaaaat
      481 tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt
      541 cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      601 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
      661 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
      721 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
      781 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat
      841 cccggacacc atcgaatggc gcaaaacctt tcgcggtagc
//
