LOCUS       sequence-50240-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 1569
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..81)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(110..820)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(190..213)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(388..407)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     657..675
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             827..838
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
       61 ctgagccttt cgttttattt gatgcctcta gcacgcgtac catgggccct tacttgtaca
      121 gctcgtccat gccgccggtt gagtggcggc cctcggcgcg ctcgtactgt tccacgatgg
      181 tgtagtcctc gttgtgggag gtgatgtcca gcttgatgtt gacgttgtag gcgccgggaa
      241 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt
      301 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
      361 gctcggagga ggcctcccag cccattgtct tcttctgcat tacggggccg tcggagggga
      421 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
      481 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
      541 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
      601 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg
      661 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
      721 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
      781 tgatgatggc catgttatcc tcctcgccct tgctcaccat cgtacgtttc tcctctttaa
      841 taagcttctg gataaggatt atccaattct aaaaaaaa
//
