LOCUS       sequence-50246-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS8
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             575..592
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(645..663)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      659..706
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(830..849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcctctga atattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgctccaga
      121 gattatcgac agaagaagct acgaggtggg cagattcctt tgatgtgctt ctctctcata
      181 agtatggggt ggctgcattc cgtgccttct tgaagacgga gttcagtgag gagaacctgg
      241 aattctggtt ggcctgtgag gagttcaaga agaccaggtc cactgcaaaa ctggtctcta
      301 aggcccatag gatctttgag gagtttgtgg atgtgcaggc tccacgggag gtaaacattg
      361 acttccagac ccgagaagcc acgaggaaga acctgcagga gccatccctg acttgctttg
      421 accaagccca aggaaaagta cacagcctca tggagaaaga ctcttacccc aggttcctga
      481 ggtccaaaat gtacttagat ctgctgtcct gacagtaaag gtggatacgg atccgaattc
      541 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      601 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      661 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      721 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      781 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      841 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      901 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgata
//
