LOCUS       sequence-50248-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX7
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(578..598)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(623..640)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             651..673
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    688..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(712..731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(815..834)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgttacaccg gatctcantg gtggtggtgg tggtgctcga gtgcggccgc aancttgtcg
       61 acggagctcg aattcggatc cgtatccacc tttactgtca cctcacgagc gctgtgatct
      121 ggggtctgac ctcctccact gacacagttg ggtcccaagc ccctaccacc tttccatctg
      181 gggctactag gtacttccag aagttccagg tgggctcctt cccagaagtc tgggccaggt
      241 acttgaaggc aggatgggca ccagtaccgg tgactgcaat cttgctaaac atggggaatg
      301 agacactgta ggtgcggcgg gcaaagctct caatctcctt gttgctgtca ggctcctgtt
      361 ggccaaactg gttgcagggg aaggcgagca cgttgaagtg gtgggggccc aggtctcgct
      421 gcagctgctg cagggctcgg tagtgctggt ctgtgaagcc gcactcgctg gccacattca
      481 ccaccaggga caccgatccg cggtacttct ccagcgacac cagtttgccc cggatgttga
      541 ccgccttgaa gtcgtagaag tcctgctcct gctgcatgga ttggaagtac aggttctcgg
      601 tacccagatc tacaccagaa gaatgatgat gatgatggtg catatgtata tctccttctt
      661 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
      721 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc
      781 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      841 gaagaatcgg gctcgccact tcgggctcat ga
//
