LOCUS       sequence-50251-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL601
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     196..214
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      601..686
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    743..759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccaccttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
       61 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
      121 accttaccag agggcgcccc agctggcaat tccgacgtct aagaaaccat tattatcatg
      181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagtcccta
      241 tcagtgatag agattgacat ccctatcagt gatagagata ctgtcgacat agagcgagga
      301 cgctaacagg aacaatgact caggatgagg gtcaggagcg ccaggaggcg aagacagagg
      361 attgtcagga agacaaacgt ccggagacgt aattaaacgg aaatggaatc aacacggatt
      421 gttccggcta aaggaaaaac agggtgtgtt ggcggcctgc aaggattgta agacccgtta
      481 agggttatga gtcaggaaaa aaggcgacag agtaatctgt cgcctttttt ctttgcttgc
      541 tttctgttag attccgccgc aaatctatac tggggcccat ggtacgcgtg ctagaggcat
      601 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      661 gtgaacgctc tcctgagtag gacaaaancc gccgccctag acctaggcgc ggatccataa
      721 atgtgagcgg ataacattga cattgtgagc ggataacaag atactcccgg gtaaggagga
      781 aaaaaaatgc tgattctgac tcgtcgagtt ggtgagaccc tcatgattgg ggatgaggtc
      841 accgtgacag ttttaggggt aaagggcaac
//
