LOCUS       sequence-50254-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RACGAP1
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             780..797
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(850..868)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      864..912
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgggaattcc tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggag
      121 ggaatgctgg cagactttgt gtcccagact tctccaatga tcccctccat tgttgtgcat
      181 tgtgtaaatg agattgagca aagaggtctg actgagacag gcctgtatag gatctctggc
      241 tgtgaccgca cagtaaaaga gctgaaagag aaattcctca gagtgaaaac tgtacccctc
      301 ctcagcaaag tggatgatat ccatgctatc tgtagccttc taaaagactt tcttcgaaac
      361 ctcaaagaac ctcttctgac ctttcgcctt aacagagcct ttatggaagc agcagaaatc
      421 acagatgaag acaacagcat agctgccatg taccaagctg ttggtgaact gccccaggcc
      481 aacagggaca cattagcttt cctcatgatt cacttgcaga gagtggctca gagtccacat
      541 actaaaatgg atgttgccaa tctggctaaa gtctttggcc ctacaatagt ggcccatgct
      601 gtgcccaatc cagacccagt gacaatgtta caggacatca agcgtcaacc caaggtggtt
      661 gagcgcctgc tttccttgcc tctggagtat tggagtcagt tcatgatggt ggagtgacag
      721 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      781 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      841 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cggggtcttg
      901 aggggttttt tgctgaaagg gaggaactat atccggattg gcgaatggg
//
