LOCUS       sequence-50255-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GRASP
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             456..473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(526..544)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      540..587
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(711..730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgcagcagcg gaaagtgctg
      121 acgttggaga aggaggataa ccagaccttc ggctttgaga tccagactta tggccttcac
      181 caccgggagg agcagcgtgt ggaaatggtg acctttgtct gccgagttca tgagtctagc
      241 cctgcccagc tggctgggct cacaccaggg gacaccatcg ccagcgtcaa tggcctgaat
      301 gtggaaggca tccggcatcg agagattgtg gacatcatta aggcgtcagg caatgttctc
      361 agactggaaa ctctatatag ttctactttg tgacagtaaa ggtggatacg gatccgaatt
      421 cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      481 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      541 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      601 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
//
