LOCUS       sequence-50258-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM1
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(376..396)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(421..438)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             449..471
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    486..510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(510..529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(511..529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(613..632)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcttgtagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcacaagt cagattcatg
      121 ttcagatctg gctacagtga gagtcaagtt gtctgtgcag cctttgattc tgttctgagc
      181 ttccaagtgt gtcatattgc tagtattttc cccatcaatg gctgtgatta catctccaat
      241 acataaatta gctagagccg ccttgcttcc aggagtgacc cgggaaatgg cgagaggctg
      301 ctcgaagtcc ttgccgccca cgaggcggaa gccccacggc cccgggccct ggaggtctat
      361 ctgctgggtg gtcatggatt ggaagtacag gttctcggta cccagatcta caccagaaga
      421 atgatgatga tgatggtgca tatgtatatc tccttcttaa agttaaacaa aattatttct
      481 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga
      541 tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      601 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
      661 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
      721 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
      781 tactactggg ctgcttccta atgcaggagt
//
