LOCUS       sequence-50261-e         548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 548)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM2
FEATURES             Location/Qualifiers
     source          1..548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             364..381
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(434..452)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      448..495
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggcgttga cggtggatgt
       61 ggccgggcca gcgccctggg gcttccgtat cacagggggc agggatttcc acacgcccat
      121 catggtgact aaggtggccg agcggggcaa agccaaggac gctgacctcc ggcctggaga
      181 cataatcgtg gccatcaacg gggaaagcgc ggagggcatg ctgcatgccg aggcccagag
      241 caagatccgc cagagcccct cgcccctgcg gctgcagctg gaccggatca cttctttgta
      301 acagtaaagg tggatacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc
      361 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      421 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      481 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
      541 gtagcggc
//
