LOCUS       sequence-50262-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM2
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(371..391)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(416..433)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    481..505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(505..524)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(506..524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(608..627)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcctttgggc ttgtagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tccgtatcca cctttactgt tacaaagaag
      121 tgatccggtc cagctgcagc cgcaggggcg aggggctctg gcggatcttg ctctgggcct
      181 cggcatgcag catgccctcc gcgctttccc cgttgatggc cacgattatg tctccaggcc
      241 ggaggtcagc gtccttggct ttgccccgct cggccacctt agtcaccatg atgggcgtgt
      301 ggaaatccct gccccctgtg atacggaagc cccagggcgc tggcccggcc acatccaccg
      361 tcaacgccat ggattggaag tacaggttct cggtacccag atctacacca gaagaatgat
      421 gatgatgatg gtgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
      481 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
      541 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      601 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      661 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      721 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      781 ctgggctgct tcctaatgca ggagtcgcat aa
//
