LOCUS       sequence-50266-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS7
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(547..567)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(592..609)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             620..642
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    657..681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(681..700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(682..700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(784..803)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgtagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc aagatgttct
      121 gcgatccatt gagtttccag actttttctt tgcctgtaga agctcctgat aggcactgga
      181 tcttataaaa cgtgggtatg aatcactttt catcagtttg taaatgtgct cctgagcatc
      241 ttcaaatgtg tatcgtccag gttccttcac gttatgtgtg gttttgtcat aactcttgga
      301 atccaagtta atagcactgg gggctccggg agccagaaac tcttgccata tttcctgaac
      361 tcttgagggt acttctttaa taggcctctt tttcaggtcc tccactgcca gccagaatct
      421 taaattttcc gagctgaatt ctgactctag aaatttaagg aactgttctc tcccaactgg
      481 gtctttcaat gcctcgtcca tgccaaaacc ccatcgtttt accctctgct ggctcggttc
      541 tttcatggat tggaagtaca ggttctcggt acccagatct acaccagaag aatgatgatg
      601 atgatggtgc atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa
      661 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct
      721 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
      781 gcgcctatat cgccgacatc accgatgggg aagatcgggg ctcgccactt cggg
//
