LOCUS       sequence-50269-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RRAS
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..42
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             648..665
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(720..738)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggaccc cccgcccagc
       61 gagacacaca agctggtggt cgtgggcggc ggcggcgtgg gcaagagcgc gctgaccatc
      121 cagttcatcc agtcctactt cgtgtctgac tacgacccca ctattgagga ctcctacacg
      181 aagatctgca gtgtggatgg catcccagcc cggctggaca tcctggacac cgcgggccag
      241 gaagagttcg gggccatgag agagcagtac atgcgtgctg gccacggctt cctgctggtg
      301 ttcgccatta acgaccggca gagtttcaac gaggtgggca agctcttcac gcagattctg
      361 cgggtcaagg accgcgacga cttccccgtt gtgttggtcg ggaacaaggc agatctggag
      421 tcacagcgcc aggtcccccg atcagaagcc tctgccttcg gcgcctccca ccacgtggcc
      481 tactttgagg cctcggccaa actgcgtctc aacgtggacg aggcttttga gcagctggtg
      541 cgggctgtcc ggaaatacca ggaacaagag ctcccaccga gctgacagta aaggtggata
      601 cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc
      661 accactgaga tccggctgct aacaaaagcc cgaaaggaag ctgaatttgg ctgctgccac
      721 cgctgagcaa taactagcat aa
//
