LOCUS       sequence-50271-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RRAS
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(587..607)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(632..649)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             660..682
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttgtcgacgg agctcgaatt cggatccgta tccaccttta ctgtcagctc ggtgggagct
       61 cttgttcctg gtatttccgg acagcccgca ccagctgctc aaaagcctcg tccacgttga
      121 gacgcagttt ggccgaggcc tcaaagtagg ccacgtggtg ggaggcgccg aaggcagagg
      181 cttctgatcg ggggacctgg cgctgtgact ccagatctgc cttgttcccg accaacacaa
      241 cggggaagtc gtcgcggtcc ttgacccgca gaatctgcgt gaagagcttg cccacctcgt
      301 tgaaactctg ccggtcgtta atggcgaaca ccagcaggaa gccgtggcca gcacgcatgt
      361 actgctctct catggccccg aactcttcct ggcccgcggt gtccaggatg tccagccggg
      421 ctgggatgcc atccacactg cagatcttcg tgtaggagtc ctcaatagtg gggtcgtagt
      481 cagacacgaa gtaggactgg atgaactgga tggtcagcgc gctcttgccc acgccgccgc
      541 cgcccacgac caccagcttg tgtgtctcgc tgggcggggg gtccatggat tggaagtaca
      601 ggttctcggt acccagatct acaccagaag aatgatgatg atgatggtgc atatgtatat
      661 ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg cttcacaaat
      721 tcccctatag tgaagtcgta attaaatttc gcgggatcga gatctcgaat cctctacgcc
      781 ggga
//
