LOCUS       sequence-50272-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL662
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     199..217
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    238..256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    263..281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(452..470)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     720..739
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccaccttg nntcanncat gcgaaacgat cctcatcctg tctcttgatc agatcttgat
       61 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
      121 ccaaccttac cagagggcgc cccagctggc aattccgacg tctaagaaac cattattatc
      181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtcc
      241 ctatcagtga tagagattga catccctatc agtgatagag atactgtcga ctaaggagga
      301 aaaaaaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      361 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      421 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      481 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      541 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      601 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      661 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      721 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      781 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
//
