LOCUS       sequence-50273-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RRAS2
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(695..713)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgc aggagaagta
       61 ccggctcgtg gtggtcggcg ggggcggcgt gggcaagtcg gcgctcacca tccagttcat
      121 ccagtcctat tttgtaacgg attatgatcc aaccattgaa gattcttaca caaagcagtg
      181 tgtgatagat gacagagcag cccggctaga tattttggat acagcaggac aagaagagtt
      241 tggagccatg agagaacagt atatgaggac tggcgaaggc ttcctgttgg tcttttcagt
      301 cacagataga ggcagttttg aagaaatcta taagtttcaa agacagattc tcagagtaaa
      361 ggatcgtgat gagttcccaa tgattttaat tggtaataaa gcagatctgg atcatcaaag
      421 acaggtaaca caggaagaag gacaacagtt agcacggcag cttaaggtaa catacatgga
      481 ggcatcagca aagattagga tgaatgtaga tcaagctttc catgaacttg tccgggttat
      541 caggaaattt caagagcagt gacagtaaag gtggatacgg atccgaattc gagctccgtc
      601 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      661 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      721 cttggggcct cctaaacggg gtcttgtggg ggttttttgc tgaaagggag gaactatatc
      781 cggattggcg aatggggacg cgcccctggt agcggc
//
