LOCUS       sequence-50274-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RRAS2
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(561..581)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(606..623)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             634..656
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    671..695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(695..714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(696..714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttgtcgacg gagctcgaat tcggatccgt atccaccttt actgtcactg ctcttgaaat
       61 ttcctgataa cccggacaag ttcatggaaa gcttgatcta cattcatcct aatctttgct
      121 gatgcctcca tgtatgttac cttaagctgc cgtgctaact gttgtccttc ttcctgtgtt
      181 acctgtcttt gatgatccag atctgcttta ttaccaatta aaatcattgg gaactcatca
      241 cgatccttta ctctgagaat ctgtctttga aacttataga tttcttcaaa actgcctcta
      301 tctgtgactg aaaagaccaa caggaagcct tcgccagtcc tcatatactg ttctctcatg
      361 gctccaaact cttcttgtcc tgctgtatcc aaaatatcta gccgggctgc tctgtcatct
      421 atcacacact gctttgtgta agaatcttca atggttggat cataatccgt tacaaaatag
      481 gactggatga actggatggt gagcgccgac ttgcccacgc cgcccccgcc gaccaccacg
      541 agccggtact tctcctgcat ggattggaag tacaggttct cggtacccag atctacacca
      601 gaagaatgat gatgatgatg gtgcatatgt atatctcctt cttaaagtta aacaaaatta
      661 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
      721 cgggatcgag atctcgatcc tctacgccgg acgcatcgtg ggcccggcat cacccggcgc
      781 ccacagg
//
