LOCUS       sequence-50278-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DIRAS1
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             758..775
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(828..846)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      842..890
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gccggaacag agtaacgatt
      121 accgcgtggt ggtgttcggg gcgggcggcg tgggcaagag ctcgctggtg ctgcgcttcg
      181 tgaagggcac gttccgcgac acctacatcc ccaccatcga ggacacctac cggcaggtga
      241 tcagctgcga caagagcgtg tgcacgctgc agatcacaga caccaccggc agccaccagt
      301 tcccggccat gcagcgcctg tccatctcca agggccacgc cttcatcctg gtgttctccg
      361 tcaccagcaa gcagtcgctg gaggagctgg ggcccatcta caagctcatc gtgcagatca
      421 agggcagcgt ggaggacatc cccgtgatgc tcgtgggcaa caagtgtgat gagacgcagc
      481 gggaggtgga cacgcgcgag gcgcaggcgg tggcccagga gtggaagtgc gctttcatgg
      541 agacctcggc caagatgaac tacaacgtca aggagctctt ccaggagctg ctgacgctgg
      601 agacgcgccg gaacatgagc ctcaacatcg acggcaagcg ctccgggaag cagaagagga
      661 cagaccgcgt caagggcaaa tgcaccctca tgtgacagta aaggtggata cggatccgaa
      721 ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      781 tccggctgct aacaaagccc gaaaggaagc tgatgtggct gctgccaccg ctgagcaata
      841 actagcataa cccctttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      901 aactatatcc gat
//
