LOCUS       sequence-50279-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DIRAS1
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(700..720)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(745..762)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             773..795
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    810..834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(834..853)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(835..853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcacatga gggtgcattt
      121 gcccttgacg cggtctgtcc tcttctgctt cccggagcgc ttgccgtcga tgttgaggct
      181 catgttccgg cgcgtctcca gcgtcagcag ctcctggaag agctccttga cgttgtagtt
      241 catcttggcc gaggtctcca tgaaagcgca cttccactcc tgggccaccg cctgcgcctc
      301 gcgcgtgtcc acctcccgct gcgtctcatc acacttgttg cccacgagca tcacggggat
      361 gtcctccacg ctgcccttga tctgcacgat gagcttgtag atgggcccca gctcctccag
      421 cgactgcttg ctggtgacgg agaacaccag gatgaaggcg tggcccttgg agatggacag
      481 gcgctgcatg gccgggaact ggtggctgcc ggtggtgtct gtgatctgca gcgtgcacac
      541 gctcttgtcg cagctgatca cctgccggta ggtgtcctcg atggtgggga tgtaggtgtc
      601 gcggaacgtg cccttcacga agcgcagcac cagcgagctc ttgcccacgc cgcccgcccc
      661 gaacaccacc acgcggtaat cgttactctg ttccggcatg gattggaagt acaggttctc
      721 ggtacccaga tctacaccag aagaatgatg atgatgatgg tgcatatgta tatctccttc
      781 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      841 gtgagtcgta ttaatttcgc gggaatcgag atctcgatcc tctacgccgg a
//
