LOCUS       sequence-50280-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DIRAS2
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..43
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgagta acgattaccg
       61 ggtggccgtg tttggggctg gcggtgttgg caagagctcc ctggtgttga ggtttgtgaa
      121 aggcacattc cgggagagct acatcccgac ggtggaagac acctaccggc aagtgatcag
      181 ctgtgacaag agcatatgca cattgcagat caccgacacg acggggagcc accagttccc
      241 ggccatgcag cggctgtcca tctccaaagg gcacgccttc atcctggtgt actccattac
      301 cagccgacag tccttggagg agctcaagcc catctacgaa caaatctgcg agatcaaagg
      361 ggacgtggag agcatcccca tcatgctggt ggggaacaag tgtgatgaga gccccagccg
      421 cgaggtgcag agcagcgagg cggaggcctt ggcccgcaca tggaagtgtg ccttcatgga
      481 gacctcagcc aagctcaacc ataacgtgaa ggagcttttc caggagctgc tcaacctgga
      541 gaagcgcagg accgtgagtc tccagtgaca gtaaaggtgg atacggatcc gaattcgagc
      601 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgatttg gctgctgcca ccgctgagca ataactagca
      721 taaccccctt ggggcctcta aaacgggtct ttg
//
