LOCUS       sequence-50281-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DIRAS2
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(637..657)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(682..699)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             710..732
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    747..771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(771..790)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(772..790)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atactcttcg gcttgtagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcactggag
      121 actcacggtc ctgcgcttct ccaggttgag cagctcctgg aaaagctcct tcacgttatg
      181 gttgagcttg gctgaggtct ccatgaaggc acacttccat gtgcgggcca aggcctccgc
      241 ctcgctgctc tgcacctcgc ggctggggct ctcatcacac ttgttcccca ccagcatgat
      301 ggggatgctc tccacgtccc ctttgatctc gcagatttgt tcgtagatgg gcttgagctc
      361 ctccaaggac tgtcggctgg taatggagta caccaggatg aaggcgtgcc ctttggagat
      421 ggacagccgc tgcatggccg ggaactggtg gctccccgtc gtgtcggtga tctgcaatgt
      481 gcatatgctc ttgtcacagc tgatcacttg ccggtaggtg tcttccaccg tcgggatgta
      541 gctctcccgg aatgtgcctt tcacaaacct caacaccagg gagctcttgc caacaccgcc
      601 agccccaaac acggccaccc ggtaatcgtt actcatggat tggaagtaca ggttctcggt
      661 acccagatct acaccagaag aatgatgatg atgatggtgc atatgtatat ctccttctta
      721 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      781 agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccgga
//
