LOCUS       sequence-50282-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GEM
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..112
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             745..762
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(815..833)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      829..876
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat atacatatgc accatcatca
       61 tcatcattct tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgtccac
      121 agactcagtc atctcctctg agtcagggaa cacctactac cgagtggtgc tcatagggga
      181 gcagggggtg ggcaagtcca ctctggccaa catctttgca ggtgtgcatg acagcatgga
      241 cagcgactgc gaggtgctgg gagaagatac atatgaacga accctgatgg ttgatgggga
      301 aagtgcaacg attatactcc tggatatgtg ggaaaataag ggggaaaatg aatggctcca
      361 tgaccactgc atgcaggtcg gggacgcata cctgattgtc tactcaatca cagaccgagc
      421 gagcttcgag aaggcatctg agctgcgaat ccagctccgc agggcccggc agacagagga
      481 cattcccata attttggttg gcaacaaaag tgacttagtg cggtgccgag aagtgtctgt
      541 atcagaaggg agagcctgtg cagtggtgtt tgactgcaag ttcatcgaga cctctgcagc
      601 tgtccagcac aacgtgaagg agctgtttga gggcattgtg cgacaggtgc gccttcggcg
      661 ggacagcaag gagaagaatt gacagtaaag gtggatacgg atccgaattc gagctccgtc
      721 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      781 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      841 cttggggcct ctaaacgggg tcttgagggg tttttttgct gaaaggagga actatatccg
      901 gattggccga atggg
//
