LOCUS       sequence-50283-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GEM
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(679..699)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(724..741)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             752..774
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    789..813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(813..832)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(814..832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctttgggct tgtagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc aattcttctc
      121 cttgctgtcc cgccgaaggc gcacctgtcg cacaatgccc tcaaacagct ccttcacgtt
      181 gtgctggaca gctgcagagg tctcgatgaa cttgcagtca aacaccactg cacaggctct
      241 cccttctgat acagacactt ctcggcaccg cactaagtca cttttgttgc caaccaaaat
      301 tatgggaatg tcctctgtct gccgggccct gcggagctgg attcgcagct cagatgcctt
      361 ctcgaagctc gctcggtctg tgattgagta gacaatcagg tatgcgtccc cgacctgcat
      421 gcagtggtca tggagccatt cattttcccc cttattttcc cacatatcca ggagtataat
      481 cgttgcactt tccccatcaa ccatcagggt tcgttcatat gtatcttctc ccagcacctc
      541 gcagtcgctg tccatgctgt catgcacacc tgcaaagatg ttggccagag tggacttgcc
      601 caccccctgc tcccctatga gcaccactcg gtagtaggtg ttccctgact cagaggagat
      661 gactgagtct gtggacatgg attggaagta caggttctcg gtacccagat ctacaccaga
      721 agaatgatga tgatgatggt gcatatgtat atctccttct taaagttaaa caaaattatt
      781 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      841 gacgagactc gatctcac
//
