LOCUS       sequence-50287-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RAC3
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(653..673)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(698..715)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             726..748
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    763..787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(787..806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(788..806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(891..910)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atacttcttt gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt
       61 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg tatccacctt tactgtcatc
      121 cgagcaccgc gcggatcgcc tcgtcaaaca ctgtcttcag gccccgctgg gtcagggctg
      181 agcactccag gtatttcaca gagccaatct cccgggccat ggccaggccc tgtgggtagg
      241 tgatgggtgc cagcttcttg tcccgcagcc gctcaatggt gtccttgtcg tcgcggaggt
      301 ccagcttggt gcccaccagg aggatgggcg tgtgggggca gtggtgccgc acctccgggt
      361 accacttggc acgaacattc tcgaaggagg ccgggctcac cagagagaag cagatcagaa
      421 agacgtcagt ttgggggtag gagagtggcc gcagccgatc gtagtcctcc tgacccgctg
      481 tgtcccacag ccccaagttg actggtttcc cgtccaccat cacgttggca gagtagttgt
      541 caaaaacggt ggggatgtac tctccgggga aggcgttggt cgtgtagctg atcagcaagc
      601 atgtcttccc cacggcgccg tcgccgacca ccacgcactt gatggcctgc atggattgga
      661 agtacaggtt ctcggtaccc agatctacac cagaagaatg atgatgatga tggtgcatat
      721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      781 caattcccct atagtgagtc gtattaattt cgcggggatc gagatctcga tcctctacgc
      841 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      901 cgacatcacc gatggggaaa aatcggggct cgccacttcg gggctcatga
//
