LOCUS       sequence-50288-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GAN
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(480..500)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(525..542)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             553..575
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    590..614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(614..633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(615..633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(717..736)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttctttgggc ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcattccaaa
      121 aactcacagc acagggtttt aaggtccgtc agtagcagca ggtcagctgc ctgaacaaca
      181 tcttggattg tatcttcatt tagcctgatc tgcccactga agatgtaatc caggatctct
      241 ctcataacca ttaccgatat cccttcaagt tcaatcttat aagttgatcc atcatctttt
      301 ggaggattat agtttaactt tgtcctgatg tacgggctgg ccgccgccag gatgttcttc
      361 tgcaccggga tctcctcccc gtcgaggacc aggtgcgcgt cgcagaagcg agactcctcg
      421 cggaaagagc tgagcgctcg cagcagacgc gcggcgtgct gagggtcaga cacggccatg
      481 gattggaagt acaggttctc ggtacccaga tctacaccag aagaatgatg atgatgatgg
      541 tgcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      601 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      661 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      721 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      781 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      841 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgttcagg
//
