LOCUS       sequence-50294-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DUSP13
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             576..593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             643..660
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(713..731)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 acctccggag gcccaagatc catggggcag tccaggcatc tccctaccag ccgcccacat
       61 tggcttcgct gcagcgcttg ctgtgggtcc gtcaggctgc cacactgaac catatcgatg
      121 aggtctggcc cagcctcttc ctgggagatg cgtacgcagc ccgggacaag agcaagctga
      181 tccagctggg aatcacccac gttgtgaatg ccgctgcagg caagttccag gtggacacag
      241 gtgccaaatt ctaccgtgga atgtccctgg agtactatgg catcgaggcg gatgacaacc
      301 ccttcttcga cctcagtgtc tactttctgc ctgttgctcg atacatccga gctgccctca
      361 gtgttcccca aggccgcgtg ctggtacact gtgccatggg ggtaagccgc tctgccacac
      421 ttgtcctggc cttcctcatg atctatgaga acatgacgct ggtagaggcc atccagacgg
      481 tgcaggccca ccgcaatatc tgccctaact caggcttcct ccggcagctc caggttctgg
      541 acaaccgact ggggcgggag acggggcggt tcgcgcacca tcatcaccac cattgaggat
      601 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
      661 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      721 caataactag cataaccccc ttggggcctc taaacgggtc ttgagggttt ttttgctgaa
      781 agggaggaac tatatccgga atggcgaatg ggaacgcgcc ctgta
//
