LOCUS       sequence-50307-e        2171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_wt_Venus_173
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             84..788
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
     CDS             1368..2102
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     1505..1526
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1629..1650)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1890..1909)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggttcagga tccgtgagca agggcgagga gaccacaatg ggcgtaatca
      121 agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac gccttcgtga
      181 tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac ctggaggtga
      241 aggagggagc ccccctgccc ttctcctacg acattctgac caccgcgttc gcctacggca
      301 acagggcctt caccaagtac cccgacgaca tccccaacta cttcaagcag tccttccccg
      361 agggctactc ttgggagcgc accatgacct tcgaggacaa gggcatcgtg aaggtgaagt
      421 ccgacatctc catggaggag gactccttca tctacgagat acacctcaag ggcgagaact
      481 tcccccccaa cggccccgtg atgcagaaga agaccaccgg ctgggacgcc tccaccgaga
      541 ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg
      601 gcggcggcca ccaccgcgtt gacttcaaga ccatctacag ggccaagaag gcggtgaagc
      661 tgcccgacta tcactttgtg gaccaccgca tcgagatcct gaaccacgac aaggactaca
      721 acaaggtgac cgtttacgag agcgccgtgg cccgcaactc caccgacggc atggacgagc
      781 tgtacaagtc cggaatggcg gacgaggaga agctgccgcc cggctgggag aagcgcatga
      841 gccgcagctc aggccgagtg tactacttca accacatcac taacgccagc cagtgggagc
      901 ggcccagcgg caacagcagc agtggtggca aaaacgggca gggggagcct gccaggaagc
      961 tgcgcattct gcagtcgact agggagcaag ggcttgtgcc cgggtctaga ggcggaggtg
     1021 gtggaggagg tggaggtggt ggaggtggag gtggaggtgg tggcggtggt ggaggaggag
     1081 gagctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg
     1141 gcggtggtgg aggaggagga gccagcggcg gaggtggtgg aggaggtgga ggtggtggag
     1201 gtggaggtgg aggtggtggc ggtggtggag gaggaggaac cggtgctgac caactgactg
     1261 aagagtggtc gacggtacag cggaccgcgc ctgacgtacc acgtacacca gtaggaaagg
     1321 caaagctttc attccaattc ccgcgggcgc gggacatcgc ggccgcgatg gacggcggcg
     1381 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1441 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1501 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1561 tgtacaaggg tggcagcggt ggcatggtga gcaagggcga ggagctgttc accggggtgg
     1621 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1681 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     1741 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg atgtgcttcg
     1801 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1861 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1921 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1981 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2041 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     2101 agggtaccga tatccttcaa aaaaaacttg aagaacttga acttgacgaa tgagatatct
     2161 aatgactcga g
//
