LOCUS       sequence-50308-e        2171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_wt_Venus_195
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             84..788
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
     primer_bind     1442..1463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(1521..1523,1524..1526,1527..2039)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1566..1587)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1827..1846)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggttcagga tccgtgagca agggcgagga gaccacaatg ggcgtaatca
      121 agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac gccttcgtga
      181 tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac ctggaggtga
      241 aggagggagc ccccctgccc ttctcctacg acattctgac caccgcgttc gcctacggca
      301 acagggcctt caccaagtac cccgacgaca tccccaacta cttcaagcag tccttccccg
      361 agggctactc ttgggagcgc accatgacct tcgaggacaa gggcatcgtg aaggtgaagt
      421 ccgacatctc catggaggag gactccttca tctacgagat acacctcaag ggcgagaact
      481 tcccccccaa cggccccgtg atgcagaaga agaccaccgg ctgggacgcc tccaccgaga
      541 ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg
      601 gcggcggcca ccaccgcgtt gacttcaaga ccatctacag ggccaagaag gcggtgaagc
      661 tgcccgacta tcactttgtg gaccaccgca tcgagatcct gaaccacgac aaggactaca
      721 acaaggtgac cgtttacgag agcgccgtgg cccgcaactc caccgacggc atggacgagc
      781 tgtacaagtc cggaatggcg gacgaggaga agctgccgcc cggctgggag aagcgcatga
      841 gccgcagctc aggccgagtg tactacttca accacatcac taacgccagc cagtgggagc
      901 ggcccagcgg caacagcagc agtggtggca aaaacgggca gggggagcct gccaggaagc
      961 tgcgcattct gcagtcgact agggagcaag ggcttgtgcc cgggtctaga ggcggaggtg
     1021 gtggaggagg tggaggtggt ggaggtggag gtggaggtgg tggcggtggt ggaggaggag
     1081 gagctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg
     1141 gcggtggtgg aggaggagga gccagcggcg gaggtggtgg aggaggtgga ggtggtggag
     1201 gtggaggtgg aggtggtggc ggtggtggag gaggaggaac cggtgctgac caactgactg
     1261 aagagtggtc gacggtacag cggaccgcgc ctgacgtacc acgtacacca gtaggaaagg
     1321 caaagctttc attccaattc ccgcgggcgc gggacatcgc ggccgccatg ctgctgcccg
     1381 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1441 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1501 acaagggtgg cagcggtggc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1561 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1621 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     1681 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgatg tgcttcgccc
     1741 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1801 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1861 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1921 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1981 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     2041 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2101 tgggtaccga tatccttcaa aaaaaacttg aagaacttga acttgacgaa tgagatatct
     2161 aatgactcga g
//
