LOCUS       sequence-50311-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RNGTT
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..44
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             814..831
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tactttaaga aggagatata catatgcacc atcatcatca tcattcttct ggtgtagatc
       61 tgggtaccga gaacctgtac ttccaatcca tggctcacaa caagatcccg ccgcggtggc
      121 tgaactgtcc ccggcgcggc cagccggtgg caggaagatt cttacctctg aagacaatgt
      181 taggaccaag atatgatagt caagttgctg aagaaaatcg gttccatccc agcatgctct
      241 caaattacct aaagagccta aaggttaaaa tgggcttgtt ggtggacctg acaaatactt
      301 caaggttcta tgaccgaaat gacatagaaa aagaaggaat caaatatata aaacttcagt
      361 gtaaaggaca tggtgagtgc cctaccactg agaatactga gacctttatt cgtctgtgtg
      421 agcggtttaa tgaaagaaat ccacctgaac ttataggtgt tcattgtact catggcttca
      481 atcgcactgg tttcctcata tgtgcctttt tggtggagaa aatggattgg agtatcgaag
      541 cagcagttgc tacttttgcc caagccagac caccaggaat ctacaagggt gattatttga
      601 aggaactttt tcgtcggtat ggtgacatag aggaagcacc acccccacct ctattgccag
      661 attggtgttt tgaggatgat gaagacgaag atgaggatga ggatggaaag aaggaatcag
      721 aaaccgggtc aagtgcttct tttggctaag acagtaaagg tggatacgga tccgaattcg
      781 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      841 gctgctaaca aagcccgaaa ggaagctga
//
