LOCUS       sequence-50312-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RNGTT
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(764..784)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(809..826)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcttagcca aaagaagcac
      121 ttgacccggt ttctgattcc ttctttccat cctcatcctc atcttcgtct tcatcatcct
      181 caaaacacca atctggcaat agaggtgggg gtggtgcttc ctctatgtca ccataccgac
      241 gaaaaagttc cttcaaataa tcacccttgt agattcctgg tggtctggct tgggcaaaag
      301 tagcaactgc tgcttcgata ctccaatcca ttttctccac caaaaaggca catatgagga
      361 aaccagtgcg attgaagcca tgagtacaat gaacacctat aagttcaggt ggatttcttt
      421 cattaaaccg ctcacacaga cgaataaagg tctcagtatt ctcagtggta gggcactcac
      481 catgtccttt acactgaagt tttatatatt tgattccttc tttttctatg tcatttcggt
      541 catagaacct tgaagtattt gtcaggtcca ccaacaagcc cattttaacc tttaggctct
      601 ttaggtaatt tgagagcatg ctgggatgga accgattttc ttcagcaact tgactatcat
      661 atcttggtcc taacattgtc ttcagaggta agaatcttcc tgccaccggc tggccgcgcc
      721 ggggacagtt cagccaccgc ggcgggatct tgttgtgagc catggattgg aagtacaggt
      781 tctcggtacc cagatctaca ccagaagaat gatgatgatg atggtgca
//
