LOCUS       sequence-50318-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1318
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..729
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(179..198)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctcgagcacc gtcgttgttg acatttttat
       61 gcttggcggt tataatggat tccacacacc cgggtaagga ggaaaatgtc tagattagat
      121 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
      181 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
      241 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
      301 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
      361 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
      421 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
      481 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
      541 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
      601 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
      661 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
      721 gggtcttaag ctttaatcgt acagggtagt acaaataaaa aaggcacgtc agatgacgtg
      781 ccttttttct tgtgagcagt
//
