LOCUS       sequence-50324-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 279
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     523..542
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    639..655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cactttccat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
       61 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      121 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
      181 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
      241 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      301 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      361 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      421 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      481 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      541 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      601 atctaatcta gacattttcc tccttacccg ggagtatctt gttatccgct cacaatgtca
      661 atgttatccg ctcacattta tggatcctga gcggatacat atttgaatgt atttagaaaa
      721 ataaacaaaa gagtttgtag aaacgcaaaa aggccatccg tcaggatgg
//
