LOCUS       sequence-50325-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BACH1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(412..432)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(457..474)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             485..507
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    522..546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(546..565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(547..565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(649..668)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgacggagct cgaattcgga tccgtatcca cctttactgt caaaatttca gaaactgaaa
       61 gcaggattcc tcaatattat gtacacttaa aaactccaca catttgcaca cttcatccac
      121 attctcttta cttaaaatca gtttagcagt gtaggcaaac tgaattaaag gttcaaatcc
      181 tttaactgtc acctcttctg gaagagtaat gttcagctct ccatcagcct ggcctacgat
      241 tcttgagtgg aagtaactgc tgcatgccgc cagcacggac cggtgagcgc ggaaccgctg
      301 tccctccaca aagatggtga catcgcacag cacatctttc ttccgctggt cattaaggct
      361 gagtaaaaca ttggtgctat gcacagaaga ttcataggca aaaaccgaca tggattggaa
      421 gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat ggtgcatatg
      481 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      541 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      601 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      661 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      721 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      781 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      841 aggagtcgca taagggaga
//
