LOCUS       sequence-50326-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACADVL
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gtcctttgct gtgggaatgt
      121 tcaaaggcca gctcaccaca gatcaggtgt tcccataccc gtccgtgctc aacgaagagc
      181 agacacagtt tcttaaagag ctggtggagc ctgtgtcccg tttcttcgag gaagtgaacg
      241 atcccgccaa gaatgacgct ctggagatgg tggaggagac cacttggcag ggcctcaagg
      301 agctgggggc ctttggtctg caagtgccca gtgagctggg tggtgtgggc ctttgcaaca
      361 cccagtacgc ccgtttggtg gagatcgtgg gcatgcatga ccttggcgtg ggcattaccc
      421 tgggggccca tcagagcatc ggtttcaaag gcatcctgct ctttggcaca aaggcccaga
      481 aagaaaaata cctccccaag ctggcatctg gggagactgt ggccgctttc tgtctaaccg
      541 agccctcaag cgggtcagat gcagcctcca tccgaacctc tgctgtgccc agcccctgtg
      601 gaaaatacta taccctcaat ggaagcaagc tttggatcag taatgggggc ctagcagaca
      661 tcttcacggt ctttgccaag acaccagtta cagatccagc cacaggagcc gtgaaggaga
      721 agatcacagc ttttgtggtg gagaggggct tcgggggcat tacccatggg ccccctgaga
      781 agaagatggg catcaaggct tcaaacacag cagaggtgtt ctttgatgga gtacggt
//
