LOCUS       sequence-50333-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 1569
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    147..163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(256..283)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(375..461)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     614..631
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(749..769)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttctggatag gattatccaa ttctaaaaaa aaagcccgga cgactgttcg ggcttgtctt
       61 tttatatgtt ggaaaatcag tggcaatgca atggcccaac agaaaataaa atctgtgaca
      121 ataaaggcat ataacccggg agtatcttgt tatccgctca caatgtcaat gttatccgct
      181 cacatttatg gatcctgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaaaga
      241 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg
      301 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa
      361 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
      421 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac
      481 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
      541 catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttatcagacc
      601 gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca
      661 gccaagctag cattaaactg ctgcagcgta gttttcgtcg tttgctgctt tgtatagttc
      721 atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca tgtggtctct
      781 cttttcgttg
//
