LOCUS       sequence-50339-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 1569
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(85..102)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      255..341
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      433..460
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    553..569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             724..735
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tatacaagca gcaaacgacg aaaactacgc tgcagcagtt taatgctagc ttggctgttt
       61 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
      121 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
      181 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
      241 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      301 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
      361 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
      421 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
      481 tgtttatttt tctaaataca ttcaaatatg tatccgctca ggatccataa atgtgagcgg
      541 ataacattga cattgtgagc ggataacaag atactcccgg gttatatgcc tttattgtca
      601 cagattttat tttctgttgg gccattgcat tgccactgat tttccaacat ataaaaagac
      661 aagcccgaac agtcgtccgg gctttttttt tagaattgga taatccttat ccagaagctt
      721 attaaagagg agaaacgtac gatggtgagc aagggcgagg aggataacat ggccatcatc
      781 aag
//
