LOCUS       sequence-50357-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ECHS1
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tggcctcggg tgctaacttt
      121 gagtacatca tcgcagaaaa aagagggaag aataacaccg tggggttgat ccaactgaac
      181 cgccccaagg ccctcaatgc actttgcgat ggcctgattg acgagctcaa ccaggccctg
      241 aagatcttcg aggaggaccc ggccgtgggg gccattgtcc tcaccggcgg ggataaggcc
      301 tttgcagctg gagctgatat caaggaaatg cagaacctga gtttccagga ctgttactcc
      361 agcaagttct tgaagcactg ggaccacctc acccaggtca agaagccagt catcgctgct
      421 gtcaatggct atgcctttgg cgggggctgt gagcttgcca tgatgtgtga tatcatctat
      481 gccggtgaga aggcccagtt tgcacagccg gagatcttaa taggaaccat cccaggtgcg
      541 ggcggcaccc agagactcac ccgtgctgtt gggaagtcgc tggcgatgga gatggtcctc
      601 accggtgacc ggatctcagc ccaggacgcc aagcaagcag gtcttgtcag caagatttgt
      661 cctgttgaga cactggtgga agaagccatc cagtgtgcag aaaaaattgc cagcaattct
      721 aaaattgtag tagcgatggc caaagaatca gtgaatgcag cttttgaaat gacattaaca
      781 gaaggaagta agttggagaa gaaactcttt tattcaacct ttgccactga tgaccggaaa
      841 gaagggatga ccgcgtttgt ggaaaagaga aaggccaact tcaaagacca gtaacagtaa
      901 aggtggaata cggatccgaa at
//
