LOCUS       sequence-50365-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TP53I3
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgttagccgt gcactttgac
      121 aagccgggag gaccggaaaa cctctacgtg aaggaggtgg ccaagccgag cccgggggag
      181 ggtgaagtcc tcctgaaggt ggcggccagc gccctgaacc gggcggactt aatgcagaga
      241 caaggccagt atgacccacc tccaggagcc agcaacattt tgggacttga ggcatctgga
      301 catgtggcag agctggggcc tggctgccag ggacactgga agatcgggga cacagccatg
      361 gctctgctcc ccggtggggg ccaggctcag tacgtcactg tccccgaagg gctcctcatg
      421 cctatcccag agggattgac cctgacccag gctgcagcca tcccagaggc ctggctcacc
      481 gccttccagc tgttacatct tgtgggaaat gttcaggctg gagactatgt gctaatccat
      541 gcaggactga gtggtgtggg cacagctgct atccaactca cccggatggc tggagctatt
      601 cctctggtca cagctggctc ccagaagaag cttcaaatgg cagaaaagct tggagcagct
      661 gctggattca attacaaaaa agaggatttc tctgaagcaa cgctgaaatt caccaaaggt
      721 gctggagtta atcttattct agactgcata ggcggatcct actgggagaa gaacgtcaac
      781 tgcctggctc ttgatggtcg atgggttctc tatggtctga tgggaggagg tgacatcaat
      841 gggcccctgt tttcaaagct actttttaag cgaggaagtc tgatcaccag tttgctga
//
