LOCUS       sequence-50371-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PRDX4
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(682..702)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(727..744)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             755..777
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    792..816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(816..835)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(817..835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttccttcggg cttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcaattcag
      121 tttatcgaaa tacttcagct ttccagctgg atctgggatt attgtttcac taccaggttt
      181 ccagccagca gggcagactt ctccgtgttt gtcagtgtac tggaatgctt gaaccaaacg
      241 tagtgtctca tccactgatc tacccacagg aagatcattc agagtaattt gtcttaggat
      301 tcctttgtca tcaataatga agagacctct aagagtgtgg cctgagtcct ctaggtatac
      361 accatagtcc tttgagatct gatgggtcaa atctgaaaga agtggaatcc ttattggccc
      421 aagtcctcct tgtcttcgag gggtattaat ccaggccaaa tgggtaaact gtgaatcaac
      481 agagcatgct accacttcag tatttataga tctgaattct tcaagtctgt cgccaaaagc
      541 gataatttca gttggacaca caaatgtgaa atcaagtggg tagaagaaga aaaccaagta
      601 tttcccacga taatcagtta acttcagctc cttaaattct ccatcgatca cagctgttcc
      661 ttcccagtag ggcgctggca tggattggaa gtacaggttc tcggtaccca gatctacacc
      721 agaagaatga tgatgatgat ggtgcatatg tatatctcct tcttaaagtt aaacaaaatt
      781 atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc
      841 gcgggatcg
//
