LOCUS       sequence-50377-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B8
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(80..103)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(104..121)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(122..142)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaanct tgtcgacgga
       61 gctcgaattc ggatcctcac ttatcgtcgt catccttgta atcatggtgg tgatgatggt
      121 gcgattggaa gtagaggttc tctgccatga aaagacctcc agtgacttcc actgaggtcc
      181 ctgtgatgta tccactatct tcagatgcca agaatgcgac cacatctgcc acatcctcag
      241 ggtcccccaa gtgtcccatc gggatcattt cagtaatctt gtccaccact ttctgtggca
      301 ctttctgtgt catgggtgtt gcaatgaacc ctgggaggac agagttacag cggatcccat
      361 gtcgtccaag ctcccgggct gcggtctggg tcagcccaat cactccagcc ttggatgctg
      421 catagtttgt ctgccccacg ttccccacct ttcctacgat gctactgatg ttgatgatgg
      481 aaccacgaca accattggac accagggctt gtgctgcagc ctgagtgact aggaaggtgc
      541 ccttgaggtt gacagctatg actttgtccc agtcatcctc agacatgtgc agcagaaact
      601 catcctgggt gatgcccgca caggacacaa cgacagatgg tgggcgagaa aagcaggcct
      661 gcacttgttc cagcaggcac ctggcggccc tggcctcaga cacgtcagcc tggaaggcag
      721 catggttccc tcggggcggc ccctccttgc tccctggccc gcccagcagc cgcaccgtct
      781 cctgtgccgc tgcccggtcc aggtcgcagg cagctacggt ggccccctct ccggccaggc
      841 gtacactgac cgctcggccg atgccgctcc ccgcacctgt gaccaa
//
